Micro-patterning of mammalian cells on suspended MEMS resonant sensors for long-term growth measurements

作者：Corbin Elise A; Dorvel Brian R; Millet Larry J; King William P; Bashir Rashid^*

来源：Lab on a Chip, 2014, 14(8): 1401-1404.

DOI：10.1039/c3lc51217g

MEMS resonant mass sensors can measure the mass of individual cells, though long-term growth measurements are limited by the movement of cells off the sensor area. Micro-patterning techniques are a powerful approach to control the placement of individual cells in an arrayed format. In this work we present a method for micro-patterning cells on fully suspended resonant sensors through select functionalization and passivation of the chip surface. This method combines high-resolution photolithography with a blanket transfer technique for applying photoresist to avoid damaging the sensors. Cells are constrained to the patterned collagen area on the sensor by pluronic acting as a cell adhesion blocker. This micro-patterning method enables long-term growth measurements, which is demonstrated by a measurement of the change in mass of a human breast cancer cell over 18 h.

• 出版日期2014