Differentiation Potentials of Canine Bone Marrow Mesenchymal Stem Cells

作者：Tharasanit Theerawat^*; Phutikanit Nawapen; Wangdee Chalika; Soontornvipart Kumpanart; Tantrajak Sasijaras; Kaewamatawong Theerayuth; Suwimonteerabutr Junpen; Supaphol Pitt; Techakumphu Mongkol

来源：Thai Journal of Veterinary Medicine, 2011, 41(1): 79-86.

• bone marrow
• canine
• mesenchymal stem cells

Mesenchymal stem cells (MSCs) are multipotent cells that have characteristics of self-renewal and differentiation into various specific cell types, in particular mesodermal lineages. This study aimed at isolating, identifying and examining the differentiation capability of canine MSCs. Bone marrow aspirates were obtained from 4 dogs. Putative MSCs were then cultured in MSC medium and subpassaged. At the 3(rd) to 5(th) passages, MSCs were examined for their morphology and doubling time. Two cell lines were examined for the expression of CD 34, CD 44 and CD 90, using flow cytometry. The in vitro differentiation of these MSCs into mesodermal lineages (bone, cartilage and adipose tissues) and ectodermal lineage (neuron) was performed using osteogenic, chondrogenic, adipogenic and neurogenic media, respectively. Histological examinations (Von Kossa and alcian blue staining) and mRNA expressions (GLA and COL1A1) were used to examine the bone and cartilage differentiation, while Oil red 0 staining was used to determine adipogenic differentiation.
Plastic-adhered MSCs had high potential for cell division, with a mean doubling time of 35.4 +/- 9.3 hours. These fibroblast-like MSCs expressed MSCs markers (CD 44 and CD 90), while fewer than 5% of these MSCs were tested positive to a hemopoietic stern cell marker (CD34). Based on the histological examinations and gene expressions, these cells demonstrated the ability to differentiate into bone, cartilage and adipose tissues. In conclusion, MSCs can be isolated from canine bone marrow and these cells are capable of in vitro differentiation into specific mesodermal lineages.

• 出版日期2011-3