科研之友
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摘要
The downregulation of microRNA-26a (miR-26a) has been reported in numerous types of cancer, but its detailed functional role in cervical cancer is not yet clear. In the present study, the expression of miR-26a in human cervical cancer was confirmed and its contribution to cervical cancer progression was investigated. The expression of miR-26a was determined by reverse transcription quantitative polymerase chain reaction in human cervical tissues and cell lines. Cell growth and invasion were detected by cell counting kit-8, colony-forming assays and transwell assays following restoration of miR-26a expression. Protein tyrosine phosphatase type IVA 1 (PRL-1) was further validated as a target of miR-26a by a functional luciferase assay and western blot analysis. In addition, the overexpression of miR-26a in tumor formation in SCID mice was investigated in vivo, and the association between miR-26a and PRL-1 was assayed by Pearson's correlation coefficient. First, it was identified that miR-26a was significantly downregulated in cervical cancer compared with the paired adjacent tissues. Forced expression of miR-26a suppressed cell proliferation and invasion in vitro and inhibited the growth of tumor xenografts in vivo. PRL-1 was determined as a novel target for miR-26a and knockdown of PRL-1 partially phenocopied the effect of miR-26a restoration. In addition, PRL-1 expression was inversely correlated with miR-26a expression in cervical cancer tissues. In conclusion, the results demonstrated the role of miR-26a in cervical cancer pathogenesis and suggest it may be used as a potential novel therapeutic strategy for cervical cancer.
- 出版日期2014-9
- 单位复旦大学
