A nomenclature for intestinal in vitro cultures

作者：Stelzner Matthias^*; Helmrath Michael; Dunn James C Y; Henning Susan J; Houchen Courtney W; Kuo Calvin; Lynch John; Li Linheng; Magness Scott T; Martin Martin G; Wong Melissa H; Yu Jian

来源：American Journal of Physiology - Gastrointestinal and Liver Physiology, 2012, 302(12): G1359-G1363.

DOI：10.1152/ajpgi.00493.2011

• cell culture
• small intestine
• colon
• stem cells
• crypt
• intestinal organoid

Stelzner M, Helmrath M, Dunn JC, Henning SJ, Houchen CW, Kuo C, Lynch J, Li L, Magness ST, Martin MG, Wong MH, Yu J. A nomenclature for intestinal in vitro cultures. Am J Physiol Gastrointest Liver Physiol 302: G1359-G1363, 2012. First published March 29, 2012; doi: 10.1152/ajpgi.00493.2011.-Many advances have been reported in the long-term culture of intestinal mucosal cells in recent years. A significant number of publications have described new culture media, cell formations, and growth patterns. Furthermore, it is now possible to study, e. g., the capabilities of isolated stem cells or the interactions between stem cells and mesenchyme. However, at the moment there is significant variation in the way these structures are described and named. A standardized nomenclature would benefit the ability to communicate and compare findings from different laboratories using the different culture systems. To address this issue, members of the NIH Intestinal Stem Cell Consortium herein propose a systematic nomenclature for in vitro cultures of the small and large intestine. We begin by describing the structures that are generated by preparative steps. We then define and describe structures produced in vitro, specifically: enterosphere, enteroid, reconstituted intestinal organoid, induced intestinal organoid, colonosphere, colonoid, and colonic organoid.

• 出版日期2012-6
• 单位河北医科大学