B1b lymphocyte-derived antibodies control Borrelia hermsii independent of Fc alpha/mu receptor and in the absence of host cell contact

作者：Colombo Matthew J; Abraham David; Shibuya Akira; Alugupalli Kishore R^*

来源：Immunologic Research, 2011, 51(2-3): 249-256.

DOI：10.1007/s12026-011-8260-8

• B1b cells
• IgM
• Complement
• Borrelia hermsii
• Fc alpha/mu R

The critical role of IgM in controlling pathogen burden has been demonstrated in a variety of infection models. In the murine model of Borrelia hermsii infection, IgM is necessary and sufficient for the rapid clearance of bacteremia. Convalescent, but not na < ve, B1b cells generate a specific IgM response against B. hermsii, but the mechanism of IgM-mediated protection is unknown. Here, we show that neither Fc alpha/mu R, a high-affinity receptor for IgM, nor IgM-dependent complement activation is required for controlling B. hermsii. Bacteria in diffusion chambers with a pore size impermeable to cells were killed when diffusion chambers were implanted into either convalescent or passively immunized mice. Furthermore, adoptively transferred convalescent B1b cells in Rag1(-/-) mice produced specific IgM that also cleared B. hermsii in diffusion chambers independent of complement. These results demonstrate that IgM-mediated clearance of B. hermsii does not require opsonophagocytosis and indicate that a mechanism for in vivo B1b cell-mediated protection is through the generation of bactericidal IgM.

• 出版日期2011-12