Determination of brominated phenols in water samples by on-line coupled isotachophoresis with capillary zone electrophoresis

作者：Knob Radim^*; Marak Jozef; Stanova Andrea; Maier Vitezslav; Kaniansky Dusan; Sevcik Juraj

来源：Journal of Chromatography A, 2010, 1217(20): 3446-3451.

DOI：10.1016/j.chroma.2010.03.006

• Brominated phenols
• Capillary isotachophoresis
• Capillary electrophoresis
• On-line preconcentration
• Column-coupling electrophoresis

A method for determination of nine brominated phenols as environmental risk compounds was developed by on-line coupled capillary isotachophoresis and capillary zone electrophoresis (ITP-CZE). For ITP step, 1 x 10(-2) mol L(-1) hydrochloric acid with 3 x 10(-2) mol L(-1) ammediol pH 9.1 was used as the leading electrolyte, and 3 x 10(-2) mol L(-1) beta-alanine with 2 x 10(-2) mol L(-1) sodium hydroxide pH 10.05 was used as the terminating electrolyte. As the background electrolyte for CZE separation, 2.5 x 10(-2) mol L(-1) beta-alanine with 2.5 x 10(-2) mol L(-1) lysine pH 9.6 was used. All electrolytes contained 0.05% or 0.1% (m/v) hydroxyethylcellulose to suppress the electroosmotic flow. UV detection at wavelength 220nm was used. Detection limits in order of tens of nmol L(-1) were achieved. Good repeatability of migration times (less than 0.33% RSD) and good repeatability of peak areas (less than 7.19% RSD) at concentration level 5 x 10(-8) mol L(-1) were observed. Developed ITP-CZE method was applied to determination of brominated phenols in spiked tap and river water samples.

• 出版日期2010-5-14