Differential gene expression by fiber-optic beadarray and pathway in adrenocorticotrophin-secreting pituitary adenomas

作者：Jiang Zhi quan; Gui Song bo; Zhang Ya zhuo^*

来源：Chinese Medical Journal, 2010, 123(23): 3455-3461.

DOI：10.3760/cma.j.issn.0366-6999.2010.23.015

• pituitary adenoma
• adrenocorticotrophin adenoma
• fiber optic beadarray
• gene expression profiling
• pathway analysis

Background Adrenocorticotrophin (ACTH)-secreting pituitary adenomas account for approximately 7%-14% of all pituitary adenomas, but its pathogenesis is still enigmatic This study aimed to explore mechanisms underlying the pathogenesis of ACTH-secreting pituitary adenomas
Methods We used fiber optic beadarray to examine gene expression in three ACTH-secreting adenomas compared with three normal pituitaries Four differentially expressed genes from the three ACTH secreting adenomas and three normal pituitaries were chosen randomly for validation by reverse transcriptase-real time quantitative polymerase chain reaction (RT-qPCR) We then analyzed the differentially expressed gene profile with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway
Results Fiber optic beadarray analysis showed that the expression of 28 genes and 8 expressed sequence tags (ESTs) were significantly increased and the expression of 412 genes and 31 ESTs were significantly decreased Bioinformatic and pathway analysis showed that the genes HIGD1B, EPS8 HPGD, DAPK2, and IGFBP3 and the transforming growth factor (TGF) beta signaling pathway and extracellular matrix (ECM)-receptor interaction pathway may play important roles in tumorigenesis and progression of ACTH-secreting pituitary adenomas
Conclusions Our data suggest that numerous aberrantly expressed genes and several pathways are involved in the pathogenesis of ACTH-secreting pituitary adenomas Fiber-optic beadarray combined with pathway analysis of differential gene expression appears to be a valid method of investigating tumour pathogenesis

• 出版日期2010-12-5
• 单位北京市神经外科研究所; 首都医科大学