Nanogels fabricated from bovine serum albumin and chitosan via self-assembly for delivery of anticancer drug

作者：Wang, Yuntao; Xu, Shasha; Xiong, Wenfei; Pei, Yaqiong; Li, Bin; Chen, Yijie^*

来源：Colloids and Surfaces B: Biointerfaces , 2016, 146: 107-113.

DOI：10.1016/j.colsurfb.2016.05.043

• Bovine serum albumin
• Chitosan
• Self-assembly
• Nanogels
• Doxorubicin hydrochloride
• Anticancer

In this study, bovine serum albumin (BSA) and chitosan (CS) were used to prepare BSA-CS nanogels by a simple green self-assembly technique. Then the nanogels were successfully used to entrap doxorubicin hydrochloride (DOX) with an entrapment ratio of 46.3%, aiming to realize the slow-release effect and lower the cytotoxicity of DOX. The IC50 values of DOX-loaded BSA-CS (DOX-BSA-CS) and free DOX obtained by MTT assay in SGC7901 cells were 0.22 and 0.05 mu g/mL, respectively. The cytotoxicity of DOX significantly decreased within 24h after encapsulation by the nanogels, indicating that the loaded drug could slowly release within 24h and the BSA-CS was a good slow release system. The cellular uptake experiments indicated DOX-BSA-CS diffused faster into the cancer cell than the bare drug. The flow cytometry and TUNEL assay proved DOX-BSA-CS could induce a larger apoptosis proportion of gastric cancer cells 7901 than the bare drug and it is promising to be used for curing gastric cancer.

• 出版日期2016-10-1
• 单位华中农业大学; 湖北大学