Microanalysis of carbon monoxide in decomposed blood and hepatic tissues by headspace gas chromatography and mass spectrometry

作者：Hao Hongxia^*; Zhou Hong; Zeng Ling; Yu Zhongshan

来源：Journal of Chemical and Pharmaceutical Research, 2014, 6(6): 1709-1715.

• Carbon monoxide poisoning
• COHb
• Gas chromatography-mass spectrometry
• Headspace gas chromatography
• Headspace gas chromatography-mass spectrometry
• Hepatic tissue
• HS-GC/MS
• UV spectrophotometry

Head-Space Gas Chromatography-Mass Spectrometry (HS-GC/MS) was used to determine blood carboxyhemoglobin (COHb%), especially in cases involving blood decomposition or death, when blood extraction is impossible. Until now, the problem eluded complete solution using forensic toxicology. Therefore a method was developed using Headspace Gas Chromatography-Mass Spectrometry (HSGC/MS), which should enable the determination of COHb in decomposed blood or hepatic tissues. The technique has a clear advantage over other methods such as UV spectrophotometry. COHb% in hepatic samples were determined, which were stored at different temperatures (-20°C for 1-2 years, 0°C, and 18°C for two months). Using a column packed with molecular sieve, CO levels were quantified down to 0.01% in the air and COHb levels down to 0.2% in small blood samples quickly. The 3-min procedure requires only 0.25ml of blood sample or 1g of hepatic tissue, each time. It was shown that the method has good reproducibility with RSD of the COHb <1%. The method was accurate and reliable to investigate deaths related to CO exposure, by determining COHb and CO levels.

• 出版日期2014
• 单位中国政法大学