Mdt1 facilitates efficient repair of blocked DNA double-strand breaks and recombinational maintenance of telomeresv

作者：Pike Brietta L; Heierhorst Joerg^*

来源：Molecular and Cellular Biology, 2007, 27(18): 6532-6545.

DOI：10.1128/MCB.00471-07

DNA recombination plays critical roles in DNA repair and alternative telomere maintenance. Here we show that absence of the SQ/TQ cluster domain-containing protein Mdt1 (Ybl051c) renders Saccharomyces cerevisiae particularly hypersensitive to bleomycin, a drug that causes 3'-phospho-glycolate-blocked DNA double-strand breaks (DSBs). mdt1 Delta also hypersensitizes partially recombination-defective cells to camptothecin-induced 3'-phospho-tyrosyl protein-blocked DSBs. Remarkably, whereas mdt1 Delta cells are unable to restore broken chromosomes after bleomycin treatment, they efficiently repair "clean" endonuclease-generated DSBs. Epistasis analyses indicate that MDT1 acts in the repair of bleomycin-induced DSBs by regulating the efficiency of the homologous recombination pathway as well as telomere-related functions of the KU complex. Moreover, mdt1 Delta leads to severe synthetic growth defects with a deletion of the recombination facilitator and telomerepositioning factor gene CTF18 already in the absence of exogenous DNA damage. Importantly, mdt1 Delta causes a dramatic shift from the usually prevalent type II to the less-efficient type I pathway of recombinational telomere maintenance in the absence of telomerase in liquid senescence assays. As telomeres resemble protein-blocked DSBs, the results indicate that Mdt1 acts in a novel blocked-end-specific recombination pathway that is required for the efficiency of both drug-induced DSB repair and telomerase-independent telomere maintenance.

• 出版日期2007-9
• 单位河北医科大学