Molecular analysis and bioactivity of luteinizing hormone from Japanese eel, Anguilla japonica, produced in silkworm pupae

作者：Choi Ji Hyun; Kim Dae Jung; Hong Sun Mee^*; Jo Sun Jung; Min Kwan Sik; Sohn Young Chang; Lee Jae Man; Kusakabe Takahiro

来源：Biotechnology and Bioprocess Engineering, 2016, 21(3): 381-388.

DOI：10.1007/s12257-016-0042-7

• Japanese eel
• luteinizing hormone
• silkworm
• recombinant protein
• equine chorionic gonadotropin

Luteinizing hormone (LH), a gonadotropin hormone (GTH) of the pituitary glycoprotein family, is important in oocyte maturation, ovulation, and spermiation. In this study, we generated a Japanese eel LH (JeLH) with and without the equine chorionic gonadotropin (eCG) carboxyl-terminal peptides under the control of the polyhedron in the silkworm pupae BES to determine their expression levels, glycosylation profile, and in vitro bioactivity. The target proteins were highly expressed in the pupae hemolymph. Recombinant JeLH center dot eCG and JeLH were N- or O-glycosylated, as shown by periodic-acid Schiff staining, deglycosidase enzyme treatment, and lectin blot analyses, and showed no significant difference in the in vitro bioactivity. Both single-chain hormones and salmon pituitary extract induced maturation of Japanese eel oocytes in vitro. Recombinant LHs produced in silkworm pupae might be suitable candidates for in vivo experiments, because they can be produced in sufficient amount and can undergo N-glycosylation.

• 出版日期2016-6