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摘要
Background: Agrimonia Pllosa Ledeb (APL), a traditional Chinese medicine, has been reported a variety of biological activities, including treating T2DM. Objective: Triterpenoid compound (TC) was collected from APL. The aim of this study was to investigate the effects of TC on 3T3-L1 preadipocytes differentiation and genes related to differentiation and IR. Materials and Methods: Column chromatography was used to collect TC from ALP. 3T3-L1 cell differentiation was induced typically in the presence of various concentrations of TC or pioglitazone. Oil red 0 staining and measurement of intracellular TG content were performed on the seventh day of differentiation. Then quantitative polymerase chain reaction (O-PCR) was used to test the expressions of three transcription factors (PPAR gamma, CCAAT enhancer binding protein-alpha (C/EBP-alpha), and sterol regulatory element-binding protein 1 (SREBP-1)) and the target genes of PPAR gamma including glucose transporter (GLUT4), lipoprotein lipase (LPL), fat acid binding protein (AP2), and adiponectin in 3T3-L1 cells. Results: At the concentration of 5, 25 and 1 25 mu g/mL, TC significantly promoted triglyceride accumulation. Further study showed that TC could promote the expression of PPAR gamma, C/EBP alpha and ADD1/SREBP1 significantly at 125 mu g/mL. As for downstream genes controlled by PPAR gamma, TC at 25 and 1 25 mu g/mL could significantly promote the expression of GLUT4 and adiponectin. However, the expression of aP2 related to lipid metabolism and adiposity in the TC group was significantly lower than that in the pioglitazone group. Conclusion: TC could promote preadipocytes differentiation through activating PPAR gamma and downstream controlled genes. TC has the ideal insulin sensitization with lower adipogenic action than classical TZDs in vitro. So TC from Agrimonia Pilosa Ledeb has a good prospect as a natural drug for IR and T2DM.
- 出版日期2015-3
- 单位重庆第二师范学院; 重庆大学
