Noradrenaline modulates the presence of gonadotropin-releasing hormone in ovary. The importance of its interrelation on the ovarian steroidogenesis and apoptosis on dioestrus II in rat

作者：Bronzi Cynthia D^*; Vega Orozco Adriana S; Rodriguez Diego; Maria Rastrilla Ana; Sosa Zulema Y; Casais Marilina

来源：Journal of Steroid Biochemistry and Molecular Biology, 2015, 154: 39-46.

DOI：10.1016/j.jsbmb.2015.06.005

• GnRH
• Noradrenaline
• Coeliac ganglion
• Ovary
• Apoptosis

The aim of this work was to investigate if noradrenaline (NA), added in the coeliac ganglion -superior ovarian nerve- ovary system (CG-SON-O) and in ovary incubation, modifies the release of ovarian progesterone (P-4), gonadotropin-releasing hormone (GnRH) and oestradiol (E-2), and the expression of 3 beta-HSD and 20 alpha-HSD and proapoptotic bax and antiapoptotic bcl-2 on dioestrus II in the rat. The CG-SON-O system and the ovary were removed and placed in one cuvette containing Krebs-Ringer solution (control groups), and NA was added to the ganglion compartment in the ex vivo system and in the ovary compartment in the ovary incubation (experimental groups). P-4, GnRH and E-2 were measured by RIA, and gene expression was measured by RT-PCR. In the ex-vivo system, the release of ovarian P-4 and GnRH and the expression of 3 beta-HSD and bax decreased; E-2 and bcl-2 increased, and the bax/bcl-2 ratio decreased. However, in the ovary incubation, P-4, GnRH, the expression of 3 beta-HSD and bax increased; E-2, the expression of 20 alpha-HSD and bcl-2 decreased while the bax/bcl-2 ratio increased, thus favoring apoptosis. The peripheral nervous system protected the ovary from the apoptotic mechanisms while in the ovary incubation the effect was reverted. Our results indicate that NA regulates ovarian steroidogenesis and apoptosis by modulating GnRH release from the coeliac ganglion and ovary, being NA a possible generator of a GnRH-gonadotropins axis in the ovary. This work is expected to contribute with new evidence of the clinical importance of catecholamines and GnRH in therapy and prevention of ovarian pathologies.

• 出版日期2015-11