Non-invasive magnetic resonance imaging of oils in Botryococcus braunii green algae: Chemical shift selective and diffusion-weighted imaging

作者：van Schadewijk Remco; van den Berg Tomas E; Gupta Karthick B Sai Sankar; Ronen Itamer; de Groot Huub J M; Alia A^*

来源：PLos One, 2018, 13(8): e0203217.

DOI：10.1371/journal.pone.0203217

Botryococcus braunii is an oleaginous green algae with the distinctive property of accumulating high quantities of hydrocarbons per dry weight in its colonies. Large variation in colony structure exists, yet its implications and influence of oil distribution and diffusion dynamics are not known and could not be answered due to lack of suitable in vivo methods. This publication seeks to further the understanding on oil dynamics, by investigating naturally relevant large (700-1500 mu m) and extra-large (1500-2500 mu m) sized colonies of Botryococcus braunii (race B, strain Showa) in vivo, using a comprehensive approach of chemical shift selective imaging, chemical shift imaging and spin echo diffusion measurements at high magnetic field (17.6T). Hydrocarbon distribution in large colonies was found to be localised in two concentric oil layers with different thickness and concentration. Extra-large colonies were highly unstructured and oil was spread throughout colonies, but with large local variations. Interestingly, fluid channels were observed in extra-large colonies. Diffusion-weighted MRI revealed a strong correlation between colony heterogeneity, oil distribution, and diffusion dynamics in different parts of Botryococcus colonies. Differences between large and extra-large colonies were characterised by using T-2 weighted MRI along with relaxation measurements. Our result, therefore, provides first non-invasive MRI means to obtain spatial information on oil distribution and diffusion dynamics in Botryococcus braunii colonies.

• 出版日期2018-8-30
• 单位河北医科大学