科研之友
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摘要
A microfluidic chip manufactured from glass substrate and indium tin oxide (ITO) coated glass use for contactless conductivity detection was developed. The detecting electrodes were fabricated by screen-printing and chemical etching methods using an ITO-coated glass wafer. Then, the glass substrate containing separation channels was bonded with the bare side of the processed ITO-coated glass, thus producing an electrophoresis chip integrated with contactless conductivity detector. The prepared microchip displayed considerable stability and reproducibility. Sensitive response was obtained at optimal conditions (including the gap between electrodes, excitation frequency, and excitation voltage). The feasibility of this microfluidic device was examined by detection of inorganic ions, and further demonstrated by the quantification of aminopyrine and caffeine in a compound pharmaceutical. The two ingredients can be completely separated within 1 min. The detection limits were 814 mL(-1) and 314 mL(-1), respectively: with the correlation coefficient of 0.996-0.998 in the linear range from 10 mu g mL(-1) to 800 mu g mL(-1). The results have showed that the present method is sensitive, reliable and fast.
- 出版日期2011-10-15
- 单位中山大学
