An activity-integrated strategy involving ultra-high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry and fraction collector for rapid screening and characterization of the α-glucosidase inhibitors in Coptis chinensis Franch. (Huanglian)

作者：Ge, Ai-hua; Bai, Yang; Li, Jin; Liu, Jiao; He, Jun; Liu, Er-wei; Zhang, Peng; Zhang, Bo-li; Gao, Xiu-mei^*; Chang, Yan-xu

来源：Journal of Pharmaceutical and Biomedical Analysis, 2014, 100: 79-87.

DOI：10.1016/j.jpba.2014.07.025

• Coptis chinensis Franch. (Huanglian)
• UHPLC/Q-TOF-MS-FC
• alpha-Glucosidase inhibitors
• Alkaloids
• Phenolic acids
• Diabetes

An activity integrated strategy was established and validated to screen alpha-glucosidase inhibitors by ultra-high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry and fraction collector (UHPLC/Q-TOF-MS-FC). UHPLC was used to separate the components in Coptis chinensis Franch. (Huanglian in Chinese) extract, which was identified by UHPLC-Q-TOF-MS to acquire structural information and collected by the fraction collector. Finally, the collected fractions were tested for inhibitory activity of alpha-glucosidase. The results showed that Huanglian extract had the alpha-glucosidase inhibitory activity with the IC50 value at 3.528 mg mL(-1), which could be used for the treatment of diabetes. Alkaloids were the main components that had inhibitory activity of alpha-glucosidase in Huanglian extract, while the inhibitory activity of phenolic acids against alpha-glucosidase was relatively weaker. Coptisine, epiberberine, jatrorrhizin and berberine were screened and identified as alpha-glucosidase inhibitors from Huanglian extract in vitro. Compared with conventional methods, the integrated UHPLC/Q-TOF-MS-FC method could quantitatively analyze alpha-glucosidase inhibitory activity of individual constituent and provide the total alpha-glucosidase inhibitory activity of the samples. The results demonstrated that the activity integrated UHPLC/Q-TOF-MS-FC method was an effective and powerful tool for screening and identifying active ingredients from Traditional Chinese medicines.

• 出版日期2014-11
• 单位天津中医药大学