New Epigallocatechin Gallate (EGCG) Nanocomplexes Co-Assembled with 3-Mercapto-1-Hexanol and β-Lactoglobulin for Improvement of Antitumor Activity

作者：Yang, Yuanyuan; Jin, Peng; Zhang, Xingcai; Ravichandran, Nagaiya; Ying, Hao; Yu, Chenghuan; Ying, Huazhong; Xu, Yongquan; Yin, Junfeng; Wang, Kai; Wu, Ming; Du, Qizhen^*

来源：Journal of Biomedical Nanotechnology, 2017, 13(7): 805-814.

DOI：10.1166/jbn.2017.2400

• Epigallocatechin-3-Gallate (EGCG)
• 3-Mercapto-1-Hexanol
• Nanocomplexes
• beta-Lactoglobulin
• Tumor Inhibition

(-)-Epigallocatechin-3-gallate (EGCG), as the major catechin in green tea, is a potent antioxidant with numerous reported health benefits such as anti-proliferative and pro-apoptotic effects. Nanovehicles for EGCG delivery can effectively improve its antioxidant capacity, absorbability and bioavailability in vivo. We used thermally modified beta-lactoglobulin (beta-Lg), 3-mercapto-1-hexanol (3MH) and EGCG to form stable co-assembled nanocomplexes (ME beta-NPs) with greater stability, sustained release and anticancer effects in vitro and in vivo than free EGCG. ME beta-NPs inhibited A375, Hep G2 and TE-1 tumor cell proliferation with 65.90%, 60.44% and 32.88% greater activity, respectively, than EGCG. ME beta-NPs with 100 mg EGCG/kg (3MH/EGCG/beta-LG = 4: 32: 1) were not toxic to mice and inhibited implanted human melanoma A375 cell tumor growth by 57.78%, twice as effective as EGCG alone. Thus, ME beta-NPs have greater stability and antitumor activity than EGCG, with potential value for anticancer therapy.

• 出版日期2017-7
• 单位浙江农林大学; 浙江省医学科学院; 中国农业科学院