Sp1 mediates glucose activation of the acetyl-CoA carboxylase promoter

作者：Daniel S; Kim KH

来源：Journal of Biological Chemistry, 1996, 271(3): 1385-1392.

Acetyl-CoA carboxylase (ACC), the rate-limiting enzyme in the biosynthesis of fatty acids, is induced in the presence of high glucose levels, The ACC gene contains two promoters: promoter I (PI) expression is inducible under lipogenic conditions, while promoter II (PII) expression, even though constitutively expressed in all tissues, is also controlled under various physiological conditions, Examination of the expression pattern of a series of deletion constructs of PII showed that the region from -340 to -249 was essential for ACC induction, In addition, by electrophoretic mobility shift assays, supershift assays, and DNase I footprinting studies, we have detected the binding of the transcription factor Spl at the two GC-rich sequences located within the -340 to -249 region of promoter II, Mutations at the GC-rich sequences prevented binding of Spl, and the induction of the PII promoter was no longer observed, Cotransfection studies, in Drosophila Schneider SL2 cells, with the Spl expression vector and PII-CAT constructs, have further confirmed the activation of promoter II by Spl, In addition, we have identified Sp3, another member of the Spl family of transcription factors, as a second factor that can bind to the glucose response elements of PII.

• 出版日期1996-1-19