科研之友
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摘要
The aim of the present study was to detect Single Nucleotide Polymorphism (SNP) in some areas of Pit-1 gene of goose to find genetic marker influencing on growth and carcass trait. The Single Nucleotide Polymorphism (SNP) of Pit-1 gene promoter was investigated in Wanxi geese. The primers for promoter in Pit-1 gene were designed according to the Pit-1 gene promoter of goose and the SNPs were detected by PCR-SSCP method. The results showed that only C-930T was detected for SNP and two genotypes (CC and TT) were found, respectively. The analysis of least square on genotypes and groups showed that there were significant differences (p<0.01) on body weight of different weeks in. different groups and genotypes, however, there were no differences (p>0.05) on body weight of different weeks in genotype-group interaction effect, then, comparison of bodyweight in different groups show that the average body weight of wanxi white goose was the largest, the average body weight of taihu goose was larger than zi goose. Variance analysis on genotype suggested that the average body weight of CC genotype and TT genotype had significant differences (p<0.05) in 6, 8 weeks and had extremely significant differences (p<0.05) in 10 weeks. This study indicated that the polymorphic site could be a genetic marker for growth trait.
- 出版日期2011
- 单位扬州大学
