Determination of Fatty Acids in Saliva of Smokers and Nonsmokers by HPLC with Fluorescence Detection Using a Hydrazine-Based Difluoro-boraindacene Reagent

作者：Guo, Xiao-Feng; Li, Yun; Wang, Hong^*; Zhang, Hua-Shan

来源：Chromatographia, 2014, 77(5-6): 431-438.

DOI：10.1007/s10337-014-2627-7

• High-performance liquid chromatography
• Fluorescence detection
• Fatty acid
• 1,3,5,7-Tetramethyl-8-daminozide-difluoroboradiaza-s-indacence

1,3,5,7-Tetramethyl-8-daminozide-difluoroboradiaza- s-indacence (TMBODIPY-H), a pre-column fluorescent derivatization reagent, was applied to the analysis of fatty acid by high-performance liquid chromatography with fluorescence detection. Using this reagent, 12 fatty acids from propionic acid (C3) to stearic acid (C18) can be derivatized at room temperature in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide within 2 h. The baseline separation of these derivatives can be achieved within 46 min by gradient elution. The detection limits of these fatty acids are in the range of 0.1-1 nM and the linear ranges of most of them are 0.5-100 nM. This was the first application of hydrazine-based difluoro-boraindacene reagent for the analysis of fatty acids, and the proposed method has been successfully used for the determination of saliva samples of smokers and nonsmokers with recoveries of 88-110 %.

• 出版日期2014-3
• 单位武汉大学