Molecular characterization of a progeny between tangerines 'Clementina Fina' and 'Montenegrina'

作者：Weiler Roberto Luis; Brugnara Eduardo Cesar; Schwarz Sergio Francisco^*; Bastianel Marines; Machado Marcos Antonio; Schifino Wittmann Maria Teresa

来源：Ciencia Rural, 2010, 40(7): 1523-1529.

DOI：10.1590/S0103-84782010005000104

• Citrus
• molecular markers
• microssatelites

Citrus have a very, complex taxonomy, especially considering the number of species included in genus Citrus and related genera. What is classified as a species may have originated by interespecific hybridization and preserved through nucellar embryony. This research aimed to characterize a population of hybrid tangerines, originated from the cross of 'Clementina Fina' (Citrus clementina Hort. ex Tan.) as female and 'Montenegrina' (Citrus deliciosa Ten.) as male parents, using microsatellite molecular makers. With 12 pairs of primers it was possible to differentiate 93 of the studied accessions and to group F, individuals nearer to the male or to the female parent. The primers PIC (Polymorphism information Content) ranged from 0.27 to 0.65. All the analyzed progeny between 'Clementina Fina' and 'Montenegrina' is hybrid, where SSR were efficient in identifying hybrids more similar to the genitors, showing genetic variability among plants of the studied population.

• 出版日期2010