An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice

作者：Xu Tongtong; Yang Min; Li Yifeng; Chen Xin; Wang Qiurong; Deng Wangping; Pang Xiayan; Yu Kate; Jiang Baohong; Guan Shuhong; Guo De an^*

来源：Rapid Communications in Mass Spectrometry, 2013, 27(21): 2297-2309.

DOI：10.1002/rcm.6696

RATIONALELicorice (Gancao) is derived from the dried roots and rhizomes of Glycyrrhiza species (Leguminosae) and appears as a component herb in about 60% of traditional Chinese medicine (TCM) prescriptions. Modern pharmacological studies have shown that flavonoids are one class of the major components responsible for the bioactivities of licorice. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/QTOF MS) has proven to be a powerful tool for rapid profiling and identification of natural products in complex herbal medicines.
METHODSA UPLC/QTOF MS method was established for the first time for profiling and structural characterization of the phenolic compounds (most of them flavonoids) in licorice. The combined use of data-independent acquisition (MSE) and data-dependent acquisition (DDA) was illustrated.
RESULTSFifteen flavonoid reference compounds were used to explore the fragmentation pathways. Compound identification was based upon the exact mass, general fragmentation behaviors, retention times, UV absorption, and the related botanical biogenesis. As a result, a total of 51 compounds were characterized, three of which were reported for the first time.
CONCLUSIONSThe LC/MS analysis for each injection took less than 9 min. The developed method is fast, accurate and reliable due to its high resolution and high efficiency characteristics as a result of combining both UPLC separation and QTOF exact mass measurement.

• 出版日期2013-11-15
• 单位中国科学院上海药物研究所