A Simplified Murine Intimal Hyperplasia Model Founded on a Focal Carotid Stenosis

作者：Tao Ming; Mauro Christine R; Yu Peng; Favreau John T; Nguyen Binh; Gaudette Glenn R; Ozaki C Keith^*

来源：American Journal Of Pathology, 2013, 182(1): 277-287.

DOI：10.1016/j.ajpath.2012.10.002

Murine models offer a powerful toot for unraveling the mechanisms of intimal hyperplasia and vascular remodeling, although their technical complexity increases experimental variability and limits widespread application. We describe a simple and clinically relevant mouse model of arterial intimal hyperplasia and remodeling. Focal left carotid artery (LCA) stenosis was created by placing 9-0 nylon suture around the artery using an external 35-gauge mandrel needle (middle or distal Location), which was then removed. The effect of adjunctive diet-induced obesity was defined. Flowmetry, wall strain analyses, biomicroscopy, and histology were completed. LCA blood flow sharply decreased by similar to 85%, followed by a responsive right carotid artery increase of similar to 71%. Circumferential strain decreased by similar to 2.1% proximal to the stenosis in both dietary groups. At 28 days, morphologic adaptations included proximal LCA intimal hyperplasia, which was exacerbated by diet-induced obesity. The proximal and distal LCA underwent outward and negative inward remodeling, respectively, in the mid-focal stenosis (remodeling indexes, 1.10 and 0.53). A simple, defined common carotid focal stenosis yields reproducible murine intimal hyperplasia and substantial differentials in arterial wall adaptations. This model offers a tool for investigating mechanisms of hemodynamically driven intimal hyperplasia and arterial wall remodeling. (Am J Pathol 2013, 182: 277-287; http://dx.doi.org/10.1016/j.ajpath.2012.10.002)

• 出版日期2013-1