摘要

This research aimed to enhance the survival of Lactobacillus reuteri KUB-AC5 from heat conditioning by using microencapsulation with aluminum carboxymethyl cellulose-rice bran (AICMC-RB) composites of different weight ratios of 1:0, 1:1, and 1:1.5. The cell/polymer suspension was crosslinked with aluminum chloride at different agitation speeds of 1200, 1500, and 2100 rpm. The AICMC microcapsules had significantly higher encapsulation efficiency, but lower microcapsule yield than the AICMC-RB microcapsules (p <= 0.05). Scanning electron microscopy revealed the complexation between AICMC and RB. Fourier transform infrared spectroscopy showed hydrogen bondings between AICMC, RB, and cells. The AICMC-RB microcapsules had significantly lower aluminum ion and moisture contents than the AICMC ones. After heat exposure, the viability of non-encapsulated and microencapsulated cells in the AICMC matrix dramatically declined, while that of microencapsulated cells in the AICMC-RB matrix was about 8 log CFU/g. The results showed the promising potential of the AICMC-RB composite microcapsules for the protection of probiotics against heat.

  • 出版日期2012-9-1