An HR-MAS MR Metabolomics Study on Breast Tissues Obtained with Core Needle Biopsy

作者：Li Mulan^*; Song Yonghyun; Cho Nariya; Chang Jung Min; Koo Hye Ryoung; Yi Ann; Kim Hyeonjin; Park Sunghyouk; Moon Woo Kyung

来源：PLos One, 2011, 6(10): e25563.

DOI：10.1371/journal.pone.0025563

Background: Much research has been devoted to the development of new breast cancer diagnostic measures, including those involving high-resolution magic angle spinning (HR-MAS) magnetic resonance (MR) spectroscopic techniques. Previous HR-MAS MR results have been obtained from post-surgery samples, which limits their direct clinical applicability.
Methodology/Principal Findings: In the present study, we performed HR-MAS MR spectroscopic studies on 31 breast tissue samples (13 cancer and 18 non-cancer) obtained by percutaneous core needle biopsy. We showed that cancer and non-cancer samples can be discriminated very well with Orthogonal Projections to Latent Structure-Discriminant Analysis (OPLS-DA) multivariate model on the MR spectra. A subsequent blind test showed 69% sensitivity and 94% specificity in the prediction of the cancer status. A spectral analysis showed that in cancer cells, taurine-and choline-containing compounds are elevated. Our approach, additionally, could predict the progesterone receptor statuses of the cancer patients.
Conclusions/Significance: HR-MAS MR metabolomics on intact breast tissues obtained by core needle biopsy may have a potential to be used as a complement to the current diagnostic and prognostic measures for breast cancers.

• 出版日期2011-10-18