Multicolor Real-Time PCR Genotyping of ABO System Using Displacing Probes

作者：Ruan Li; Zhao Huian; Li Qingge^*

来源：Journal of Forensic Sciences, 2010, 55(1): 19-24.

DOI：10.1111/j.1556-4029.2009.01228.x

• forensic science
• genotyping
• ABO blood group
• real-time polymerase chain reaction
• displacing probes
• single nucleotide polymorphisms

Rapid and informative ABO genotyping has become increasingly popular in forensic use. We developed a multiplex real-time polymerase chain reaction (PCR) approach to genotype ABO major groups and subgroups. Seven differently fluorophor-labeled displacing probes for O1(261delG), A(261G), A(796C/803C), B(796A/803C), O2 (802G > A), A2 (1059delC), and A2 (1009A > G) were combined in one or two PCRs to determine either ABO major groups or subgroups. The method correctly detected 13 reference DNA samples. A blind test of 237 samples resulted in complete agreement with their phenotypes, and 110 of these 237 samples as well as with PCR-SSP method. The whole analysis could be finished in less than 100 min at substantially low material cost and the template DNA ranging from 0.16 to 500 ng per reaction could be quantitatively detected. Despite the limited informativeness of ABO genotyping, the developed methods could find application in rapid and inexpensive screening of forensic settings.

• 出版日期2010-1
• 单位厦门大学; 司法部司法鉴定科学技术研究所