Nondysplastic Ulcerative Colitis Has High Levels of the Homologous Recombination Repair Protein NUCKS1 and Low Levels of the DNA Damage Marker Gamma-H2AX

作者：De Angelis Paula M; Schjolberg Aasa R; Hughes Juliana B; Huitfeldt Henrik S; Andersen Solveig Norheim; Ostvold Anne Carine

来源：Inflammatory Bowel Diseases, 2018, 24(3): 593-600.

DOI：10.1093/ibd/izx071

• ulcerative colitis
• sporadic colorectal cancer
• NUCKS1
• gamma-H2AX (gamma H2AX)
• DNA double-strand breaks
• DNA repair protein

Background: The colon and rectum are continuously exposed to oxidative stress that generates reactive oxygen species, which are a major cause of DNA double-strand breaks (DSB). Furthermore, chronic inflammatory diseases such as ulcerative colitis (UC) are characterized by an excess of reactive nitrogen species that can also lead to DNA double-strand breakage and genomic instability. We investigated the expression of the nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1) protein in UC and sporadic colorectal cancer (CRC) due to its involvement in both DNA double-strand break repair and inflammatory signaling.
Methods: NUCKS1 expression and expression of the DNA double-strand break marker gamma-H2AX (gamma H2AX) were assessed in formalin-fixed, paraffin-embedded UC and CRC patient biopsies using peroxidase immunohistochemistry. Expression levels for both proteins were evaluated together with previously published expression-level data for hTERT and TP53 proteins in the same material.
Results: Nondysplastic UC lesions had 10-fold lower gamma H2AX expression and approximately 4-fold higher NUCKS1 expression compared with sporadic CRC, indicating minimal DNA DSB damage and heightened DNA DSB repair in these lesions, respectively. NUCKS1 expression in UC tended to decrease with increasing grades of dysplasia, whereas gamma H2AX, hTERT, and TP53 expression tended to increase with increasing grades of dysplasia. The highest gamma H2AX expression was seen in sporadic CRC, indicating considerable DNA DSB damage, whereas the highest NUCKS1 expression and hTERT expression were seen in nondysplastic UC.
Conclusions: Overall, our data suggest that NUCKS1 may be involved in DNA DSB repair and/or inflammatory signaling in UC, but a more thorough investigation of both pathways in UC is warranted.

• 出版日期2018-3