Higher cardiogenic potential of IPSCs derived from cardiac versus skin stromal cells

作者：Meraviglia Viviana; Wen Jianyan; Piacentini Luca; Campostrini Giulia; Wang Cheng; Florio Maria Cristina; Azzimato Valerio; Fassina Lorenzo; Langes Martin; Wong Johnson; Miragoli Michele; Gaetano Carlo; Pompilio Giulio; Barbuti Andrea; DiFrancesco Dario; Mascalzoni Deborah; Pramstaller Peter P; Colombo Gualtiero I; Chen Huei Sheng Vincent; Rossini Alessandra^*

来源：Frontiers in Bioscience, 2016, 21(4): 719-743.

DOI：10.2741/4417

• Induced Pluripotent Stem Cells
• Stromal Cells
• Fibroblasts
• Cardiomyogenic Differentiation
• Cardiomyocytes

Prior studies have demonstrated that founder cell type could influence induced pluripotent stem cells (iPSCs) molecular and developmental properties at early passages after establishing their pluripotent state. Herein, we evaluated the persistence of a functional memory related to the tissue of origin in iPSCs from syngeneic cardiac (CStC) vs skin stromal cells (SStCs). We found that, at passages greater than 15, iPSCs from cardiac stromal cells (C-iPSCs) produced a higher number of beating embryoid bodies than iPSCs from skin stromal cells (S-iPSCs). Flow cytometry analysis revealed that dissected beating areas from C-iPSCs exhibited more Troponin-T positive cells compared to S-iPSCs. Beating areas derived from C-iPSCs displayed higher expression of cardiac markers, more hyperpolarized diastolic potentials, larger action potential amplitude and higher contractility than beaters from skin. Also, different microRNA subsets were differentially modulated in CStCs vs SStCs during the reprogramming process, potentially accounting for the higher cardiogenic potentials of C-iPSCs vs S-iPSCs. Therefore, the present work supports the existence of a founder organ memory in iPSCs obtained from the stromal component of the origin tissue.

• 出版日期2016-1-1