科研之友
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摘要
A new biosensor was prepared based on conductive polymer poly (3,4-ethylenedioxythiophene) (PEDOT) and 1-pyrenebutanoic acid (PBA) through pi-pi stacking, as well as hematin associated with PBA through coordinate bonds of Zr4+ and carboxyl group. Its stability and sensitivity were examined by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and current. time (i-t) method. A pair of well defined and quasi. reversible redox peaks was observed when GCE/PEDOT/PBA/hematin was tested by CV in PBS without oxygen. The electron transfer rate constant was estimated to be 4-8 s(-1). Results indicated that the PEDOT film enhanced the electron transfer process of hematin. When the i-t method was used to detect the response of biosensor to catechol with different concentrations, it displayed a linear response for the reduction of catechol in the range of 0.5-200 mol/L. The linear equation was i = 0.018C + 0.006 (R = 0.9998), and the detection sensitivity was 0.258 mu A (mu mol/L.cm(2)) with a detection limit of 0.33 nmol/L (S/N = 3). The results illustrate that the GCE/PEDOT/PBA/hematin biosensor is very sensitive and stable.
