A novel lipid droplets-targeting ratiometric fluorescence probe for hypochlorous acid in living cells

作者：Wu, Wen-Li; Ma, Han-Lin; Xi, Long-Long; Huang, Miao-Fei; Wang, Kai-Ming^*; Miao, Jun-Ying; Zhao, Bao-Xiang^*

来源：Talanta, 2019, 194: 308-313.

DOI：10.1016/j.talanta.2018.10.006

• Ratiometric fluorescence
• Lipid droplets-targeting
• Endogenous hypochlorous acid
• Cell image

Lipid droplets were found to be involved in many organism activities. Here, a lipid droplets-targeted near infrared fluorescence probe (named XHZ) for ratiometric detection of endogenous hypochlorous acid/hypochlorite (HClO/ClO-) in living cells was developed, which was constructed by a coumarin moiety and a malononitrile derivative. XHZ could detect HClO/ClO- with high selectivity and sensitivity in a ratiometric manner based on FRET (Forster Resonance Energy Transfer) mechanism. The two well-resolved emission (470/672 nm) bands could ensure accurate detection of HClO/ClO- in vitro as well as in vivo. XHZ was successfully used for ratiometric fluorescence imaging of exogenous and endogenous HClO/ClO- in RAW264.7 cells. A good linear relationship between the fluorescence intensity ratios of the two emissions and HClO/ClO- concentrations from 0 to 40 mu m was obtained. Importantly, XHZ could localize mainly in lipid droplets of RAW264.7 cells. To the best of our knowledge, XHZ is the first lipid droplets-targeted ratiometric fluorescence probe for HClO/ClO-.

• 出版日期2019-3-1
• 单位济南大学; 山东大学