A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA)

作者:Kai Junhai*; Puntambekar Aniruddha; Santiago Nelson; Lee Se Hwan; Sehy David W; Moore Victor; Han Jungyoup; Ahn Chong H
来源:Lab on a Chip, 2012, 12(21): 4257-4262.
DOI:10.1039/c2lc40585g

摘要

In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.

  • 出版日期2012