Development of monoclonal antibodies against pirimiphos-methyl and their application to IC-ELISA

作者：Yang ZY; Kolosova AY; Shim WB; Chung DH^*

来源：Journal of Agricultural and Food Chemistry, 2006, 54(13): 4551-4556.

DOI：10.1021/jf0606196

• pirimiphos methyl
• pesticide
• monoclonal antibody
• immunoassay
• ELISA

To detect the organophosphorus (OP) pesticide pirimiphos-methyl in grain samples, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed and optimized. By the active esters method, pirimiphos-methyl hapten A was conjugated to keyhole limpet hemocyanin to be used as the immunogen for the production of monoclonal antibodies, and pirimiphos-methyl hapten B was conjugated to ovalbumin to be used as coating antigen. By using the monoclonal antibody and the coating antigen, an IC-ELISA has been developed. Under the established optimized conditions, the IC-ELISA showed an IC50 of 4.2 ng/mL with a detection limit of 0.07 ng/mL. The IC-ELISA showed negligible cross-reactivity with other OP pesticides except with pirimiphos-ethyl. Recoveries of pirimiphos-methyl from spiked grain samples ranged from 83 to 96%.

• 出版日期2006-6-28