Iwr1 facilitates RNA polymerase II dynamics during transcription elongation

作者：Gomez Navarro Natalia; Peiro Chova Lorena; Estruch Francisco^*

来源：Biochimica et Biophysica Acta-Gene Regulatory Mechanisms, 2017, 1860(7): 803-811.

DOI：10.1016/j.bbagrm.2017.02.009

• RNA polymerase
• Transcription elongation
• lwr1
• Genome instability
• Transcription coupled repair

Iwr1 is an RNA polymerase II (RNPII) interacting protein that directs nuclear import of the enzyme which has been previously assembled in the cytoplasm. Here we present genetic and molecular evidence that links Iwr1 with transcription. Our results indicate that Iwr1 interacts with RNPII during elongation and is involved in the disassembly of the enzyme from chromatin. This function is especially important in resolving problems posed by damage-arrested RNPII, as shown by the sensitivity of iwrl mutants to genotoxic drugs and the Iwr1's genetic interactions with RNPII degradation pathway mutants. Moreover, absence of Iwr1 causes genome instability that is enhanced by defects in the DNA repair machinery.

• 出版日期2017-7