Assessment of Correlation Between p16(INK4a) Staining, Specific Subtype of Human Papillomavirus, and Progression of LSIL/CIN1 Lesions First Comparative Study

作者：Razmpoosh Maryam; Sansregret Andree; Oligny Luc L; Patey Natalie; Dormoy Raclet Virginie; Ducruet Thierry; Soglio Dorothee Bouron Dal^*

来源：American Journal of Clinical Pathology, 2014, 142(1): 104-110.

DOI：10.1309/AJCPBX74QXCPXIKK

• Low-grade squamous intraepithelial lesion
• LSIL
• Cervical intraepithelial neoplasia grade 1
• CIN1
• p16
• Human papillomavirus
• HPV subtype
• Prognosis

Objectives: To study and compare the effectiveness of p16(INK4a) staining and specific human papillomavirus (HPV) subtypes as a prognostic marker in cervical intraepithelial neoplasia grade 1 (CIN1; low-grade squamous intraepithelial lesions).
Methods: Sixty-four cervical samples diagnosed as CIN1 and stained with p16(INK4a), with HPV status assessed by polymerase chain reaction direct sequencing.
Results: Of the 34 p16(INK4a)-negative biopsy specimens, 26 regressed, seven persisted, and one progressed Of the 20 p16(INK4a) diffusely positive biopsy specimens, seven regressed, eight persisted, and five progressed. Ten biopsy specimens stained positive only in the lower one-third of the sample, of which seven regressed and three persisted p16(INK4a) diffusely positive CIN1 lesions were associated with only high-risk HPV subtypes, with the exception of one HPV-negative biopsy specimen. Three different high-risk HPV subtypes and one low-risk HPV subtype (HPV66) were identified in the six CIN1 lesions that progressed
Conclusions: There is a significant relationship between p16(INK4a) immunostaining and follow-up (P = .002). p16(INK4a)-negative specimens or positivity in the lower one-third of CIN1 lesions seldom progress to a CIN2-3 lesion.

• 出版日期2014-7