Anti-Aging Effects of Hesperidin on Saccharomyces cerevisiae via Inhibition of Reactive Oxygen Species and UTH1 Gene Expression

作者：Sun, Kaiyue; Xiang, Lan^*; Ishihara, Satoru; Matsuura, Akira; Sakagami, Youji; Qi, Jianhua

来源：Bioscience Biotechnology and Biochemistry, 2012, 76(4): 640-645.

DOI：10.1271/bbb.110535

• hesperidin
• Sir2
• UTH1
• anti-aging
• reactive oxygen species (ROS)

This study used a replicative lifespan assay of K6001 yeast to screen anti-aging food factors in commercial flavonoids. Hesperidin derived from the Citrus genus extended the lifespan of yeast at doses of 5 and 10 mu M as compared with the control group (p < 0.01, p < 0.01). Reactive oxygen species (ROS), real-time PCR (RTPCR), and lifespan assays of uth1 and skn7 mutants with the K6001 background were used to study the anti-aging mechanisms in yeast. The results indicate that hesperidin significantly inhibits the ROS of yeast, and UTH1 gene expression, and that SKN7 gene are involved in hesperidin-mediated lifespan extension. Further, increases in the Sir2 homolog, SIRT1 activity, and SOD gene expression were confirmed at doses of 5 (p < 0.01) and 10 mu m (p < 0.05). This suggests that Sir2, UTH1 genes, and ROS inhibition after administration of hesperidin have important roles in the anti-aging effects of yeast. However, the aglycon hesperetin did not exhibit anti-aging effects in yeast.

• 出版日期2012-4
• 单位浙江大学