Generation of human induced pluripotent stem cells from oral mucosa

作者：Miyoshi Keiko; Tsuji Daisuke; Kudoh Keiko; Satomura Kazuhito; Muto Taro; Itoh Kohji; Noma Takafumi^*

来源：Journal of Bioscience and Bioengineering, 2010, 110(3): 345-350.

DOI：10.1016/j.jbiosc.2010.03.004

• Oral mucosa fibroblasts
• Induced pluripotent stem cells
• Personalized medicine
• Regenerative medicine

Induced pluripotent stem (iPS) cells are one of the most promising sources for cell therapy in regenerative medicine. Using a patient's own genetically identical and histocompatible cells is the ideal way to practice personalized regenerative medicine. For personalized iPS cell therapy, the prerequisites for cell source preparation are a simple and safe procedure, no aesthetic or functional damage, and quick wound healing. Oral mucosa fibroblasts (OFs) may have high potential to fulfill these requirements. In this study, biopsy was performed in a dental chair; no significant incisional damage was recognized and rapid wound healing (within a week) was observed. We generated human iPS cells from the isolated OFs via the retroviral gene transfer of OCT4, SOX2, c-MYC, and KLF4. Reprogrammed cells showed ES-like morphology and expressed undifferentiated markers such as OCT4, NANOG, SSEA4, TRA-1-60, and TRA-1-81. Subsequent in vitro and in vivo analyses confirmed the pluripotency of resultant iPS cells, which matched the criteria for iPS cells. In addition, we found that the endogenous expression levels of c-MYC and ICLF4 in OFs were similar to those in dermal fibroblasts. Taken together, we propose that OFs could be a practical source for preparing iPS cells to achieve personalized regenerative medicine in the near future.

• 出版日期2010-9
• 单位河北医科大学