科研之友
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摘要
A concave microchannel surface was formed by nanosecond pulse laser ablation to allow antibody immobilization on a capillary flow immunoassay chip. Microscopic analysis showed that UV laser ablation of poly(methyl methacrylate) at 193 nm and 1.76 J . cm(-2) allowed excellent immobilization of Cy5 conjugated antibody. The concave structure was 10 mu m deep and 260 mu m wide and supported uniform antibody printing on the microchannel surface. The characteristics of immobilized antibodies on this surface and on a commercially available polymer coating were comparable. Quantitative analysis of procollagen type I C-peptide (PICP) at different concentrations provided a linear relationship in the range 0-600 ng ml(-1) PICP, which is sufficient for clinical estimation of PICP in the blood. The results may provide a new benchmark for a mechatronic antibody immobilization-based capillary flow immunoassay chip.
- 出版日期2015-7
- 单位中国地震局
