Large-scale isolation of high-purity anthocyanin monomers from mulberry fruits by combined chromatographic techniques

作者：Chen, Yao; Du, Fang; Wang, Wei; Li, Qian; Zheng, Daheng; Zhang, Weijie; Zhao, Ting; Mao, Guanghua; Feng, Weiwei; Wu, Xiangyang^*; Yang, Liuqing^*

来源：Journal of Separation Science, 2017, 40(17): 3506-3512.

DOI：10.1002/jssc.201700471

• anthocyanin monomers
• column chromatography
• high-speed countercurrent chromatography
• large-scale separation
• mulberry fruits

Anthocyanins have attracted attention over the past several decades because of their beneficial health effects. In this research, a strategy combining column chromatography and high-speed countercurrent chromatography was developed for the separation of high-purity anthocyanin monomers from mulberry fruits. After purification using Amberlite XAD-7HP column with 80% ethanol (0.1% HCl), a fraction of anthocyanins mixtures with a purity of 68.6% was obtained. High-speed countercurrent chromatography with a biphasic solvent system of n-butanol/methyl tertbutyl ether/acetonitrile/water/trifluoroacetic acid (30:10:10:50:0.05, v/v) was used to separate the anthocyanin monomers. Three monomers of delphinidin-3-O-rutinoside, cyanidin-3-O-rutinoside, and cyanidin-3-O-glucoside were obtained, and identified by H-1 and C-13 NMR spectroscopy and high-performance liquid chromatography with electrospray ionization-mass spectrometry. The method developed in this work can be used to conduct large-scale separations of anthocyanin monomers from mulberry fruits and other plants.

• 出版日期2017-9
• 单位江苏大学