科研之友
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摘要
In pH 8.4 Tris-HCl buffer solutions, alcohol dehydrogenase catalyzed the reaction between ethanol and nicotinamide adenine dinucleotide to produce acetaldehyde. In the medium of HCl, acetaldehyde reduced HAuCl4 to form gold particles that exhibited a strong resonance scattering (RS) peak at 600 nm. The RS peak increased with ethanol concentration. The increased RS intensity at 600 nm (Delta I-600 nm) was proportional to the ethanol concentration (C) from 0.068 to 10.2 mmol/L, with a regression equation of Delta I-600 (nm)=035.59C + 16.1, and a detection limit (3 sigma) of 3.2 mu mol/L. This proposed method was applied to detect ethanol in saliva and plant cell culture medium samples, with satisfactory results.
