A circular cross-section PDMS microfluidics system for replication of cardiovascular flow conditions

作者：Fiddes Lindsey K; Raz Neta; Srigunapalan Suthan; Tumarkan Ethan; Simmons Craig A; Wheeler Aaron R^*; Kumacheva Eugenia

来源：Biomaterials, 2010, 31(13): 3459-3464.

DOI：10.1016/j.biomaterials.2010.01.082

• Microchannels
• Flow
• Cardiovascular system
• Circular cross-section
• Patterning
• Seeding of endothelial cells

Since the inception of soft lithography, microfluidic devices for cardiovascular research have been fabricated easily and cost-effectively using the soft lithography method. The drawback of this method was the fabrication of microchannels with rectangular cross-sections, which did not replicate the circular cross-sections of blood vessels. This article presents a novel, straightforward approach for the fabrication of microchannels with circular cross-sections in poly(dimethylsiloxane) (PDMS), using soft lithography. The method exploits the polymerization of the liquid silicone oligomer around a gas stream when both of them are coaxially introduced in the microchannel with a rectangular cross-section. We demonstrate (i) the ability to control the diameter of circular cross-sections of microchannels from ca. 40-100 mu m; (ii) the fabrication of microchannels with constrictions, and (iii) the capability to grow endothelial cells on the inner surface of the microchannels.

• 出版日期2010-5