A New Process for Acrylamide Synthesis by Enzymatic Hydrolysis of Acrylonitrile in Disperse System

作者：Tudorascu Marius; Oprea Spiridou; Marculescu Afrodita Doina; Popovici Maria; Tudorascu Stefania

来源：Revista de Chimie, 2009, 60(2): 197-200.

• Pseudomonas chlororaphis RPZ-18 strain
• nitrile hydratase activity
• amidase inactivation
• yield of acrylamide

A new process for enzymatic synthesis of acrylamide synthesis was studied. The process is characterized by using immobilized Pseudomonas chlororaphis RPZ-18 cells in a system containing concentrated solutions of acrylonitrile as substrate. Due to the limited solubility of acrylonitrile in water, the reaction system was ultrasound treated prior to the hydrolysis reaction; in this case, the reaction system is transformed into a disperse system. The bioconversion process was carried out in a wide range of substrate concentration and at different reaction temperature values. Also, the bioconversion process was entirely performed in two serially disposed enzymatic column-reactors. On this way, the obtained hydrolysis product, in our case acrylamide has a high chemical purity without non reacted substrate content.

• 出版日期2009-2
• 单位河北医科大学