科研之友
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摘要
Mast cells (MCs) are unique constituents of the human body. While inter-individual differences may influence the ways by which MCs operate in their skin habitat, they have not been surveyed in a comprehensive manner so far. We therefore set out to quantify skin MC variability in a large cohort of subjects. Pathophysiologically relevant key features were quantified and correlated: transcripts of c-kit, Fc epsilon RI alpha, Fc epsilon RI beta, Fc epsilon RI gamma, histidine decarboxylase, tryptase, and chymase; surface expression of c-Kit, Fc epsilon RI alpha; activity of tryptase, and chymase; histamine content and release triggered by Fc epsilon RI and Ca2+ ionophore. While there was substantial variability among subjects, it strongly depended on the feature under study (coefficient of variation 33-386%). Surface expression of Fc epsilon RI was positively associated with Fc epsilon RI alpha mRNA content, histamine content with HDC mRNA, and chymase activity with chymase mRNA. Also, MC signature genes were co-regulated in distinct patterns. Intriguingly, histamine levels were positively linked to tryptase and chymase activity, whereas tryptase and chymase activity appeared to be uncorrelated. Fc epsilon RI triggered histamine release was highly variable and was unrelated to Fc epsilon RI expression but unexpectedly tightly correlated with histamine release elicited by Ca2+ ionophore. This most comprehensive and systematic work of its kind provides not only detailed insights into inter-individual variability in MCs, but also uncovers unexpected patterns of co-regulation among signature attributes of the lineage. Differences in MCs among humans may well underlie clinical responses in settings of allergic reactions and complex skin disorders alike.
- 出版日期2016-6
