Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion

作者：Orlandi Sardi Janaina de Cassia; Polaquini Carlos Roberto; Freires Irlan Almeida; de Carvalho Galvao Livia Camara; Lazarini Josy Goldoni; Torrezan Guilherme Silva; Regasini Luis Octavio; Rosalen Pedro Luiz

来源：Journal of Medical Microbiology, 2017, 66(6): 816-824.

DOI：10.1099/jmm.0.000494

• diacetylcurcumin
• curcumin
• Staphylococcus aureus
• anti-adhesion
• antibiofilm
• toxicity

Purpose. Staphylococcus aureus infections have contributed to the global healthcare burden, particularly with regard to hospital-acquired meticillin-resistant S. aureus (MRSA) infections.
Methodology. This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae.
Results. DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 mu mol l(-1), and minimum bactericidal concentration ranging between 69 and 277 mu mol l(-1). It significantly reduced bacterial biofilm survival - by 22-63% (at MIC, 10 x MIC or 100 x MIC) as compared to the 25-42% reduction by vancomycin (P<0.0001) - and severely affected biofilm cell structures, leading to damaged architecture and the formation of amorphous cell clusters. Treatment with DAC (MIC/4) decreased bacterial adhesion to HaCaT keratinocytes from 1 to 5 h (P<0.0001). Finally, DAC demonstrated low toxicity in G. mellonella at its effective anti-biofilm concentrations.
Conclusion. These findings open new avenues for the study of this curcumin derivative as an excellent prototype with anti-MRSA activity.

• 出版日期2017-6