Antimicrobial Activity of Lysozyme-Functionalized Gold Nanoparticles

作者：Yang Longping; Yan Wenjing^*; Huang Mingming; Qiao Weiwei; Zhang Jianhao

来源：Shipin Kexue/Food Science, 2017, 38(14): 84-90.

DOI：10.7506/spkx1002-6630-201714013

• Anti-bacterial activity
• Anti-microbial activity
• Antibacterial mechanisms
• Antibacterial properties
• Functionalized gold nanoparticles
• Functionalized nanoparticles
• Gold Nanoparticles
• Membrane permeability

In order to improve the stability of lysozyme and its antibacterial activity against Gram-negative bacteria, as a green and highly efficient antibacterial agent, lysozyme-functionalized gold nanoparticles were prepared for the first time by surface modification of gold nanoparticles with lysozyme. The influences of the mass ratio of lysozyme to gold nanoparticles and the concentration of functionalized nanoparticles on the antibacterial activity against Gram-negative and Gram-positive bacteria were examined. The antibacterial mechanism of the prepared materials against Escherichia coli and the cytotoxicity on SH-SY5Y cells were also investigated. The results showed that compared with pure gold nanoparticles and lysozyme, the antibacterial activity of lysozyme-functionalized gold nanoparticles against E. coli and Staphylococcus aureus were obviously enhanced, which indicated the synergistic antibacterial effect of lysozyme and gold nanoparticles. Under optimized conditions, 0.1 g/L lysozyme-functionalized gold nanoparticles were able to completely kill the two types of bacteria. Besides, lysozymefunctionalized gold nanoparticles exhibited durable antibacterial properties and good biocompatibility, which were not toxic to mammalian cells. Transmission electron microscopy (TEM) images and live/dead bacterial assay showed that lysozymefunctionalized gold nanoparticles could kill bacteria by disrupting the cell membrane and cell wall.

• 出版日期2017
• 单位南京农业大学