A novel ratiometric fluorescent probe for selective detection of Hg2+, Cr3+ and Al3+ and its bioimaging application in living cells

作者:Yang, Yang; Feng, Yan; Wang, Ying-Zhe; Qiu, Fang-Zhou; Tang, Xiao-Liang; Zhang, Guo-Lin*; Liu, Wei-Sheng*
来源:Sensors and Actuators B: Chemical , 2017, 253: 1055-1062.
DOI:10.1016/j.snb.2017.07.025

摘要

A novel rhodamine-benzothiazole fluorescent probe L was designed and synthesized which can be selective detection of three kinds of metal ions (Hg2(+), Cr3(+) and Al3(+)), through the mechanism of fluorescence resonance energy transfer (FRET). This probe L shows good sensitivity towards Hg2(+), Cr3(+) and Al3(+) even at the sub-micromolar levels, which exhibited ratiometric fluorescence change from colorless to pink in aqueous media in a wide pH range of 510. Interestingly, the Jobs plot investigation suggested that L bounding to Hg2(+) or Al3(+) with a simple 1:1 mode and a 2:1 mode to Cr3(+) display different binding modes. Furthermore, the potential utility for biological applications was performed by fluorescence microscopic imaging in live cells. The results indicated that fluorescence probe L was a hopeful candidate for applications in the cell-imaging and safety inspection.

  • 出版日期2017-12
  • 单位功能有机分子化学国家重点实验室; 兰州大学