A simple method for collecting measured whole blood with quantitative recovery of antibody activities for serological surveys

作者：Handali Sukwan^*; Rodriguez Silvia; Noh John; Gonzalez Armando E; Garcia Hector H; Gilman Robert H; Roberts Jacquelin M; Hancock Kathy; Tsang Victor C W

来源：Journal of Immunological Methods, 2007, 320(1-2): 164-171.

DOI：10.1016/j.jim.2006.12.007

• finger-prick whole blood collection
• filter paper
• StabilZyme select
• FAST-ELISA

Compliance and acceptance for the finger-prick method of blood collection is generally better than for venipuncture. A fingerprick method of blood collection with quantitative antibody recovery is even more important for seroepidemiological surveys. Finger-prick blood collected and dried onto filter paper has been used, but, unfortunately, this method has several disadvantages, including loss of antibody activity, possible contact contamination from blood spots on adjacent filter papers, and difficulties in extracting antibodies, justifying the search for other methods of collecting and transporting blood samples. We report on a simple method of collecting a measured amount of finger-prick blood onto a sample pad, which is immediately transferred to storage/ extraction buffer. The diluted blood sample is never dried, and because of the storage buffer, can be transported and stored without refrigeration. Furthermore, the diluted blood samples can then be tested directly without further preparation. We systematically compared several storage/extraction buffers and commercially available filter papers. We showed that antibody recovery was not significantly affected by the type of filter papers used but was significantly affected by the storage/extraction buffer used. The best such buffer is StabilZyme Select (TM).

• 出版日期2007-3-30