A Si-rhodamine-based near-infrared fluorescent probe for visualizing endogenous peroxynitrite in living cells, tissues, and animals

作者：Miao, Junfeng; Huo, Yingying; Shi, Hu; Fang, Junru; Wang, Juanjuan; Guo, Wei^*

来源：Journal of Materials Chemistry B, 2018, 6(27): 4466-4473.

DOI：10.1039/c8tb00987b

• ISCHEMIA-REPERFUSION INJURY
• NITRIC-OXIDE
• DIABETIC-RATS
• SELECTIVE DETECTION
• TYROSINE NITRATION
• OXIDATIVE STRESS
• RENAL ISCHEMIA
• LIVE CELLS
• IN-VIVO
• ISCHEMIA/REPERFUSION INJURY

Peroxynitrite (ONOO-) is an extremely powerful biological oxidant and it can react with a wide variety of molecular targets to result in a series of disease states, which has made ONOO- a central biological pathogenic factor. In order to disclose its various pathological events, a large number of fluorescent ONOO- probes have been developed in recent years. Nevertheless, some limitations still remain, such as short excitation and emission wavelengths, long fluorescence response times, and cross-interference caused by other reactive oxygen species (ROS). In this work, a new near-infrared (NIR) fluorescent probe, SiRTA, containing an aromatic tertiary amine functional group and a Si-rhodamine fluorophore, was developed for endogenous ONOO- detection and imaging. The probe exhibits not only a fast, sensitive, and specific fluorescence off-on response toward ONOO- in chemical systems but also excellent imaging ability for endogenous ONOO- in living cells. With the probe, the therapeutic effects of phenolic acid antioxidants in EA.hy926 endothelial cells suffering from ischemia-reperfusion injury and the pathogenesis of diabetes and diabetic nephropathy in pancreatic -cells and diabetic rats have successfully been evaluated. These results indicate that SiRTA is a potentially outstanding imaging tool for studying the physiological and pathological events of ONOO- and relevant drug therapies.

• 出版日期2018-7-21
• 单位山西大学