Mannose-binding lectin as part of the complement pathway: characterization in non-inflamed and inflamed human eyes

作者：Chow Sing Pey^*; Dean Melinda M; Depla Jozef A; Daniell Mark D; Eisen Damon P

来源：Clinical and Experimental Ophthalmology, 2011, 39(9): 871-877.

DOI：10.1111/j.1442-9071.2011.02572.x

• complement pathway
• endophthalmitis
• inflammation
• mannose-binding lectin

Background: Mannose-binding lectin plays a central effector role in the lectin pathway of complement activation. Frequently occurring MBL2 polymorphisms result in mannose-binding lectin deficiency, which increases susceptibility to infection. We characterized mannose-binding lectin levels and function in non-inflamed and inflamed human eyes, and evaluated its relationship to blood mannose-binding lectin levels and function.
Design: Prospective, observational clinical study with controls and cases.
Participants: Twenty-seven patients with paired blood and ocular samples (aqueous and/or vitreous) including 15 controls (non-inflamed) and 12 cases (inflamed).
Methods: Blood and ocular samples were collected from controls (n = 15) with quiet eyes during elective cataract surgery and cases with inflamed eyes including proven/suspected endophthalmitis (n = 11) and herpetic retinal vasculitis (n = 1). Mannan-binding and C4 deposition enzyme-linked quantify mannosebinding lectin levels and function.
Main Outcome Measures: Blood and ocular mannose- binding lectin levels and function.
Results: Of 27 patients, 10 (37%) were mannosebinding lectin-deficient (defined as blood mannose- binding lectin levels < 500 ng/mL). Blood mannose-binding lectin levels (P = 0.16) or P = 0.43) were not significantly different between controls and cases. As expected, there was a high correlation between blood mannose-binding lectin levels and r(2) = 0.74). However, there was significantly more mannose-binding lectin in inflamed eyes than non-inflamed eyes measured as level (P < 0.01) or C4 deposition P < 0.01).
Conclusions: Our study demonstrated that mannosebinding lectin is significantly elevated in inflamed human eyes but virtually undetectable in noninflamed control eyes, suggesting a role in sightthreatening ocular inflammation.

• 出版日期2011-12