Anti-Salmonella Activity Modulation of Mastoparan V1A Wasp Venom ToxinUsing Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System

作者：Ha Yeon Jo; Kim Sam Woong; Lee Chae Won; Bae Chang Hwan; Yeo Joo Hong; Kim Il Suk; Gal Sang Wan; Hur Jin; Jung Ho Kyoung; Kim Min Ju; Bang Woo Young^*

来源：Toxins, 2017, 9(10): 321.

DOI：10.3390/toxins9100321

• AMP
• bacterial secretion system
• inoculum effect
• mastoparan
• MP-V1
• protease inhibitor
• Salmonella
• wasp venom toxin

A previous study highlighted that mastoparan V1 (MP-V1), a mastoparan from the venom of the social wasp Vespula vulgaris, is a potent antimicrobial peptide against Salmonella infection, which causes enteric diseases. However, there exist some limits for its practical application due to the loss of its activity in an increased bacterial density and the difficulty of its efficient production. In this study, we first modulated successfully the antimicrobial activity of synthetic MP-V1 against an increased Salmonella population using protease inhibitors, and developed an Escherichia coli secretion system efficiently producing active MP-V1. The protease inhibitors used, except pepstatin A, significantly increased the antimicrobial activity of the synthetic MP-V1 at minimum inhibitory concentrations (determined against 10(6) cfu/mL of population) against an increased population (10(8) cfu/mL) of three different Salmonella serotypes, Gallinarum, Typhimurium and Enteritidis. Meanwhile, the E. coli strain harboring OmpA SS::MP-V1 was identified to successfully secrete active MP-V1 into cell-free supernatant, whose antimicrobial activity disappeared in the increased population (10(8) cfu/mL) of Salmonella Typhimurium recovered by adding a protease inhibitor cocktail. Therefore, it has been concluded that our challenge using the E. coli secretion system with the protease inhibitors is an attractive strategy for practical application of peptide toxins, such as MP-V1.

• 出版日期2017-10