Analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS

作者：Fuchs Beate; Schiller Juergen^*; Suess Rosmarie; Zscharnack Matthias; Bader Augustinus; Mueller Peter; Schuerenberg Martin; Becker Michael; Suckau Detlev

来源：Analytical and Bioanalytical Chemistry, 2008, 392(5): 849-860.

DOI：10.1007/s00216-008-2301-8

• MALDI-TOF MS
• High Performance Thin-Layer Chromatography (HPTLC)
• Mesenchymal stem cells
• Lipids
• MS imaging
• Erythrocytes

MALDI-TOF MS is traditionally used for "proteomics", but is also a useful tool for lipid analysis. Depending on the applied matrix, however, some lipid classes are more sensitively detected than other ones and this may even lead to suppression effects if complex mixtures are analyzed. Therefore, a previous separation into the individual lipid classes is necessary. Using artificial lipid mixtures or easily available tissue extracts, it has been already shown that HPTLC-(High Performance Thin-Layer Chromatography)-separated lipids can be conveniently analyzed by MALDI-TOF MS directly on the TLC plate. Here we present an initial TLC-MALDI study of the lipid composition of ovine mesenchymal stem cells. Due to the complex composition of these cells, data are also compared to lipids extracted from human erythrocytes. It will be shown that even very minor lipid classes can be easily detected and with much higher sensitivity than by common staining protocols. Additionally, MS images of the developed TLC plates will be shown and potential applications, new methods of data analysis as well as problems discussed.

• 出版日期2008-11
• 单位河北医科大学